Can i autoclave edta

Store the sterile freezing medium at a controlled room temperature C. EDTA 0. Stir vigorously on a magnetic stirrer. Adjust the pH to 8. Adjust the volume to 1 liter with H2O. Store the NaCl solution at room temperature. Heat to 68 C, and stir with a magnetic stirrer to assist dissolution. If necessary, adjust the pH to 7. Store at room temperature.

Sterilization is not necessary. Adjust the pH to 7. Adjust the volume of the solution to 1 liter with deionized H2O. Sterilize by autoclaving for 20 minutes at 15 psi 1. Make sure that the pH of the Tris solution is 8. Lysozyme will not work efficiently if the pH of the solution is less than 8. Prepare liquid media according to the recipes given. Just before autoclaving, add one of the following:.

When the medium is removed from the autoclave, swirl it gently to distribute the melted agar or agarose evenly throughout the solution. Be careful! The fluid may be superheated and may boil over when swirled. To avoid producing air bubbles, mix the medium by swirling. Plates can then be poured directly from the flask; allow approx. To remove bubbles from medium in the plate, flame the surface of the medium with a Bunsen burner before the agar or agarose hardens.

Set up a color code e. The plates should be removed from storage hours before they are used. Alternatively, remove the liquid by shaking the lid with a single, quick motion. To minimize the possibility of contamination, hold the open plate in an inverted position while removing the liquid from the lid.

Adjust the volume to 1 liter with H2O. Usually obtained as a Adjust pH to 7. Dispense into aliquots. Dissolve g of ammonium acetate in ml of H 2 O. To 60 ml of 5 M potassium acetate add The resulting solution is 3 M with respect to potassium and 5 M with respect to acetate. Dispense into aliquots and filter sterilize. Dissolve g of NaCl, Adjust the volume to 1 liter.

Add 1 g of ethidium bromide to ml of H 2 O. Stir on a magnetic stirrer for several hours to ensure that the dye has dissolve. Ethidium bromide is a powerful mutagen. Wear gloves, a lab coat and a mask when weighing it out. Handle carefully.